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KMID : 0624620130460030163
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BMB Reports
2013 Volume.46 No. 3 p.163 ~ p.168
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Identification of a novel circularized transcript of the AML1 gene
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Xu Ai-Ning
Chen Xiu-Hua
Tan Yan-Hong
Qi Xi-Ling
Xu Zhi-Fang
Zhang Lin-Lin
Ren Fang-Gang
Bian Si-Cheng
Chen Yi
Wang Hong-Wei
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Abstract
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The AML1 gene is an essential transcription factor regulating the differentiation of hematopoietic stem cells into mature blood cells. Though at least 12 different alternatively spliced AML1 mRNAs are generated, three splice variants (AML1a, AML1b and AML1c) have been characterized. Here, using the reverse transcription-polymerase chain reaction with outward-facing primers, we identified a novel non-polyadenylated transcript from the AML1 gene, with exons 5 and 6 scrambled. The novel transcript resisted RNase R digestion, indicating it is a circular RNA structure that may originate from products of mRNA alternative splicing. The expression of thenovel transcript in different cells or cell lines of human and a number of other species matched those of the canonical transcripts. The discovery provides additional evidence that circular RNA could stably exist in vivo in human, and may also help to understand the mechanism of the regulation of the AML1 gene transcription.
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KEYWORD
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Alternative splicing, Circular RNA, Non-coding RNA, RNase R, The AML1 gene
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